1/1/2024 0 Comments Licor western blot boxesPlate Templates are used to analyze multiwell plate images in Empiria Studio. If you already have a Plate Template that can be used to analyze your protein estimation plate image, you do not need to repeat these steps. Import Zip File for AnalysisĬlick Import LI-COR Zip Files, and import the Zip File you exported from LI‑COR Acquisition Software. To analyze a protein estimation assay in Empiria Studio, follow the instructions in this section. In the Select Detection Channel dialog, select 630 Trans and click Save.Īnalyze Protein Concentration Estimation Assay in Empiria Studio® Software On the Choose Multiwell Plate Assay page, click Absorbance Assay. ![]() On the Choose Assay page, click Multiwell Plate. The Zip File can be imported into Empiria Studio for analysis.Īcquire Other Protein Estimation Assay Imagesįollow these instructions to acquire an image for the following types of protein concentration estimation assays: Bradford (Coomassie Plus) Assay, Pierce 660 Assay, Modified Lowry Assay. When the image has been acquired, click Export to Analyze. Absorbance detection is not sensitive to the focus offset value. Leave the default focus offset ( 3.00 mm). The 384 Well option has not been evaluated for protein estimation assays. On the Detection Options page, select the following options. Selecting only 525 Trans is essential for analysis in Empiria Studio. In the Select Detection Channel dialog, select 525 Trans and click Save.ĭo not select any other channel. Select the locations on the Scan Surface that have a multiwell plate to image. In the Assay list, click Absorbance Assay. ![]() Acquire Imageįollow the steps below, depending on which type of protein estimation assay you want to image. Use the Plate Alignment Guide ( 926-18972) to align plates on the Scan Surface. Place up to four multiwell plates on the Odyssey M Scan Surface. Place Multiwell Plates on the Odyssey M Imager Materials (such as from a kit) for performing one of the following assays: Micro BCA Assay, Bradford (Coomassie Plus) Assay, Pierce 660 Assay, Modified Lowry Assayįollow the manufacturer's instructions for your kit or for individual materials you will be using to prepare the assay. LI‑COR Acquisition Software version 1.0 or version 1.0.1 ( /AcquisitionQuickStart)Įmpiria Studio® Software version 2.0, version 2.1, or version 2.1.1 ( /empiria) Clear plates tend to be recommended for absorbance-based assays and will provide an image with the expected visual representation of the data. See the Odyssey M Imager manual ( /m-manual) for more information about the differences between trans illumination, epi illumination, and fluorescence detection.Ĭlear plates and black or white plates with clear bottom plates have been used for absorbance-based assays on the Odyssey M Imager. See /DetectionLabelReference for a complete list of Odyssey M Imager detection channels. ![]() The Odyssey M Imager provides three transillumination channels that are used to image absorbance-based assays: 470 Trans, 525 Trans, and 630 Trans. The following materials are required to follow the instructions in this document. The word "background" is used instead of "blank" to accommodate similar concepts across the wide variety of assays that can be analyzed in Empiria Studio. This document focuses on analysis, not experimental planning or lab protocol.īe aware that the concept called a "blank" in many protein estimation kit instructions is covered by the term "background" in Empiria Studio. Please look to the instructions for the protein estimation kit (or other materials) that you are using while planning your experiment. See for more scientific background on protein concentration estimation assays.
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